糖心vlog官网

Students and faculty access powerful lab technology thanks to new NSF grants

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Dogs are funny. Every other animal lives longer as it increases in size 鈥 mice live a year or two at most, while elephants can live for 70. Not so with dogs, whose lifespans decrease as the breeds get larger. 鈥淎 Chihuahua will live to 16 easily,鈥 says , 鈥渨hile a Great Dane is old at 6.鈥 Jimenez has been working to unravel this mystery, and she now has access to a powerful new tool to do that: a Seahorse XFe 96 oxygen flux analyzer for integrative organismal and cellular biology.

Seahorse machine with computer screen displaying sample analysis

The Ferrari on 糖心vlog官网鈥檚 benchtop

糖心vlog官网 recently acquired that mouthful of a machine through a $271,077 grant from the National Science Foundation (NSF). 鈥淚t鈥檚 our Ferrari on a benchtop,鈥 Jimenez says about the device鈥檚 high price tag, before adding: 鈥淚鈥檇 prefer the Seahorse.鈥 That鈥檚 because the state-of-the-art equipment is able to do something no other tool in the lab can do: measure metabolism inside living cells in real time. 鈥淢etabolism is the underlying biochemical process that affects everything in the cell,鈥 Jimenez says. 鈥淭his is a tool that can bring that abstract concept to life.鈥

The Seahorse is one of two recent additions to 糖心vlog官网鈥檚 equipment that juices up its already formidable capacity to carry on scientific research. 鈥淚鈥檓 not aware of any other undergraduate institution that has this equipment,鈥 says , who is using the Seahorse to investigate metabolism of viruses. 鈥淩esources like this are incredibly rare 鈥 and it鈥檚 even rarer for students to be using it to do research.鈥

Two students look down at a computer display in a lab

By learning experimentally rather than vicariously, students leave 糖心vlog官网 with all of the knowledge they need to perform high-level scientific experiments on their own.

The size of a proverbial breadbox, the Seahorse contains a plate with 96 wells in which a researcher can culture cells for an experiment. The machine can then perform an array of processes to investigate how cells use oxygen and sugars. Jimenez and her undergraduate students have already found some metabolic links to certain diseases that may affect large dogs more than smaller dogs. 鈥淥ur hope is that we might find some way to alter these metabolic pathways, possibly by altering the composition of dog food, in order to make them live longer,鈥 Jimenez says.

Holm is using the same machine to investigate how viruses alter the metabolism of human cells. 鈥淲e know viruses use a whole lot of energy, but not much is known about how they alter cells to make that energy available,鈥 he says. He is particularly interested in how viruses affect cancer cells and whether it might be possible to modify a virus to attack tumors by robbing them of oxygen while leaving healthy cells intact.

It鈥檚 not just 糖心vlog官网 faculty, however, who are investigating the role of metabolism in disease. Students in intermediate genetics class are using the Seahorse to investigate rare genetic diseases using small worms called nematodes. Each team of students chooses a human disease and analyzes its effects in the worms, which they can plop directly inside the wells of the Seahorse to measure its metabolism. 鈥淲orking in a cell culture may not allow you to understand what is happening in a whole organism,鈥 says Van Wynsberghe, whose own research involves the study of the rare disease inherited by the children and grandchildren of mothers who took the morning-sickness drug DES.

The fact that students are able to do complex science on such a sophisticated piece of equipment ramps up the level of engagement in her class, Van Wynsberghe says. 鈥淚 tell them we are going to play with a multi-hundred-thousand-dollar piece of equipment, and you can see how excited and motivated they are,鈥 she says. And by using equipment most undergrads never touch, they are able to ask much more sophisticated questions than the typical student at their level. 鈥淚t鈥檚 fantastic training in critical thinking,鈥 Van Wynsberghe says. 鈥淭hey come out of 糖心vlog官网 already thinking like a grad student.鈥

One student who has already benefited from using the equipment is Joshua Winward 鈥18, who has been working with Jimenez on researching the metabolism of dogs, driving with her for two summers to the University of Rochester, two hours each way, to perform experiments. 鈥淗aving a Seahorse on site will drastically reduce the amount of time running experiments as well as allowing us to run experiments more frequently,鈥 he says. 鈥淗aving this experience has given me an appreciation for just how much you can learn about any given organism at the cell level just by having access to the right equipment.鈥

Messing around with magnetism

Not only is 糖心vlog官网 adding new, sophisticated lab equipment to its stable, but it is also upgrading the equipment it has with new capabilities. This fall, the chemistry lab received a $345,783 grant from the NSF to improve its nuclear magnetic resonance (NMR) spectrometer, an instrument that uses the same technology as MRI machines to discover the structure of molecules. 鈥淲hen you do chemistry, you don鈥檛 know what you鈥檝e made until you run a whole lot of tests on it,鈥 says . 鈥淚t鈥檚 not like in the kitchen where you can just taste it. It becomes a puzzle to solve for things you cannot see.鈥

Two students stand on either side of the NMR machine. The student on the left is on a ladder, reaching out to adjust a component on top of the NMR.

The NMR in action (Photo by Janna Minehart)

Common techniques such as mass spectrometry only tell researchers which atoms are present, but not how they are connected 鈥 a crucial element in dealing with complex organic molecules where the position of a hydrogen or carbon atom can dramatically affect how the molecule functions. In Nolen鈥檚 research designing and constructing molecules of biomedical interest, for instance, he needs to identify a galactose sugar called TN antigen, which differs from glucose by one hydrogen atom pointing in the opposite direction. 鈥淚t鈥檚 a tiny change, but it makes a huge biological difference,鈥 he says.

The NMR consists of a powerful magnet 鈥 actually, a ball of energized superconducting wire suspended in liquid helium 鈥 that can shift the orientation that a nucleus is spinning on its axis. By detecting these 鈥渟pin flips,鈥 researchers can construct a 3-D picture of a chemical compound in real time. The new upgrade for the system will dramatically increase its sensitivity, allowing researchers to not only tell how an atom is connected to its immediate neighbor, but also how it relates to other atoms in the compound.

Sample graph rendered by 糖心vlog官网's NMR

A sample graph rendered by 糖心vlog官网鈥檚 NMR

is also using the device to characterize porphyrinoids, a versatile chemical structure in nature that does everything from binding oxygen in red blood cells and absorbing light in chlorophyll to catalyzing a range of biological reactions. Porphyrinoids can be used in a variety of applications, including harvesting energy in photovoltaic cells to improve solar energy, and destroying cancer cells by transferring energy from light to oxygen molecules. In order to achieve those goals, however, it鈥檚 necessary to discover efficient methods to synthesize these compounds 鈥 the focus of Geier鈥檚 research group. 鈥淭here is a lot of trial and error in developing practical methods to create these compounds,鈥 says Geier. 鈥淲e are seeking to uncover general principles that make the trial and error more efficient. The NMR spectrometer is a key tool for quickly determining whether we are making the compounds that we think we are.鈥

Like the Seahorse, the NMR spectrometer is also heavily used by students in both classrooms and research. , who is the chemistry department chair, uses the device in his organic chemistry lab, allowing more than 200 students per year to analyze samples. 鈥淭hey can immediately identify whether their experiments formed what they expected them to form,鈥 he says.

One of Chianese鈥檚 students, Linh Le 鈥18, is currently using the equipment in his lab to analyze reactions to create intricate catalysts called ruthenium pincer complexes. 鈥淎s an undergraduate, I feel that it is a privilege to have access to this modern equipment, which is widely used nowadays in both industry and research labs,鈥 says Le, who is in the process of applying to PhD programs in chemistry. 鈥淐onsequently, I am more prepared as I embark on my career.鈥

By learning experimentally rather than vicariously, students leave 糖心vlog官网 with all of the knowledge they need to perform high-level scientific experiments on their own. 鈥淲e can do real science,鈥 says Nolen. 鈥淲e are not just saying 鈥榟ere is an example of what you would do when you go to graduate school.鈥 We are actually doing it here.鈥

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This material is based upon work supported by the National Science Foundation under Grant Nos. 1725841 and 1726308